Sections shall be incubated with 100 μl of hybridisation buffer (4 × SSC [standard saline citrate], 50 % formamide, 1 × Denhardt's solution, 250 μg ml– 1 yeast tRNA, 10 % dextran sulphate) containing 10 ng (1 μl of the PCR reactants prepared as described in point I.3.2 using primers CCG-GTG-CCA-GGT-ATA-TCT-CG and TTC-GGG-TGG-TCT-TGA-AAG-GC) of the digoxigenin-labelled probe.
De coupes worden geïncubeerd met 100 μl of hybridisatiebuffer (4 × SSC [standaard zout-citraat], 50 % formamide, 1 × Denhardt-oplossing, 250 μg ml– 1 gist-tRNA, 10 % dextraansulfaat) dat 10 ng (1 μl van de PCR-reagentia, bereid zoals beschreven in punt I.3.2 met gebruikmaking van de primers CCG-GTG-CCA-GGT-ATA-TCT-CG en TTC-GGG-TGG-TCT-TGA-AAG-GC) van de met digoxigenin gelabelde probe bevat.
