Vertaling van "Citrate buffer solution " (Engels → Nederlands) :

TERMINOLOGIE

citrate buffer solution
citraatbufferoplossing

aqueous buffer | buffer | buffer solution
buffer | bufferoplossing

buffered solution | loading solution
bufferoplossing

Citrate anticoagulation solution
citraatoplossing tegen stolling

IN-CONTEXT TRANSLATIONS

Sections shall be incubated with 100 μl of hybridisation buffer (4 × SSC [standard saline citrate], 50 % formamide, 1 × Denhardt's solution, 250 μg ml– 1 yeast tRNA, 10 % dextran sulphate) containing 20 ng (2 μl of the PCR reaction prepared as described in point I.3.2 using primers Bo and Boas) of the digoxigenin-labelled probe.
De coupes worden geïncubeerd met 100 μl of hybridisatiebuffer (4 × SSC [standaard zout-citraat], 50 % formamide, 1 × Denhardt-oplossing, 250 μg ml– 1 gist-tRNA, 10 % dextraansulfaat) dat 20 ng (2 μl van de PCR-reagentia, bereid zoals beschreven in punt I.3.2 met gebruikmaking van de primers Bo en Boas) van de met digoxigenin gelabelde probe bevat.

Sections shall be incubated with 100 μl of hybridisation buffer (4 × SSC [standard saline citrate], 50 % formamide, 1 × Denhardt's solution, 250 μg ml– 1 yeast tRNA, 10 % dextran sulphate) containing 10 ng (1 μl of the PCR reactants prepared as described in point I.3.2 using primers CCG-GTG-CCA-GGT-ATA-TCT-CG and TTC-GGG-TGG-TCT-TGA-AAG-GC) of the digoxigenin-labelled probe.
De coupes worden geïncubeerd met 100 μl of hybridisatiebuffer (4 × SSC [standaard zout-citraat], 50 % formamide, 1 × Denhardt-oplossing, 250 μg ml– 1 gist-tRNA, 10 % dextraansulfaat) dat 10 ng (1 μl van de PCR-reagentia, bereid zoals beschreven in punt I.3.2 met gebruikmaking van de primers CCG-GTG-CCA-GGT-ATA-TCT-CG en TTC-GGG-TGG-TCT-TGA-AAG-GC) van de met digoxigenin gelabelde probe bevat.

Add 100 μl/well of chromogen/substrate solution (1 ml of ABTS (2,2'-Azino-bis-[3-ethylbenzothiazoline-6-sulphonic acid]) 5 mg/ml + 9 ml of substrate buffer (0,1 M Phosphate-Citrate buffer of pH 4 containing 0,03 % H2O2] and incubate for 10 minutes at room temperature. Colour development is stopped by adding 100 μl/well of 2 % (w/v) SDS (sodium dodecyl sulphate).
Pipetteer 100 μl chromogeen/substraatoplossing in elk putje [(1 ml ABTS (2.2'-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid]) 5 mg/ml ± 9 ml substraatbuffer (0,1M fosfaat-citraatbuffer met pH 4 die 0,03 % H2O2 bevat)] en incubeer gedurende 10 minuten bij kamertemperatuur. Stop de kleurontwikkeling door toevoeging van 2 % (w/v) SDS (natriumdodecylsulfaat) (100 μl per putje).